Faculty of Science

Objectives

To provide an in-depth coverage of the tools of molecular diagnostics: the principles and practices.

Contents

PCR-based DNA fingerprinting and satellite DNA analyses; Design of PCR primers; Design of hybridization probes; Isolation of DNA from whole blood, soft tissues, semen stains and swabs, bones, plant material; Polymorphism: Polymorphism of some genetic locus in relation to disease (HLA, Apo and ACE gene), RFLP; Applications of DNA fingerprinting; Diagnosis by multiplex PCR; ARMS-PCR, DNA barcoding, genomic southern hybridization.

Immunodiagnostics: Immunoglobins, T-cells; B cell maturation, activation and differentiation; Uses of immunoglobulins; monoclonal and polyclonal antibodies; Hybridoma technology; Labeling of antibodies; Immunotechnologies: ELISA, Western blotting, Immunochromatography, Immunohistochemistry.