Faculty of Science

Objectives

To provide an in-depth review of the available tools of immuno/DNA-based tests in diagnostic research and their usage in health care services: the principles and applications

Contents

The polymerase chain reaction; Single locus and multi-locus DNA fingerprinting; Design of PCR primers; Design of hybridization probes; Isolation of DNA from whole blood, soft tissues, semen stains and swabs, bones; Polymorphism: Polymorphism of some genetic locus in relation to disease (HLA, Apo and ACE gene); RFLP; Diagnosis by multiplex PCR; ARMS-PCR, FMR-1 gene trinucleotide repeat analysis, genomic southern hybridization. DNA bar-coding; Clinical implications and services: Synopsis of available molecular kits in healthcare and instrumentation; Procedures and results interpretation; Including molecular genetic mutations and cancer test kits, and biochemical kits. Clinical molecular tests in all areas of molecular diagnostics, including inherited conditions, molecular hematology, molecular oncology, and pharmacogenetics.