BCH709: Proteomics 6 credits (40-10-10)

Objectives

To present proteomics as a systems biology tool in identifying and characterizing proteins synthesized in a cell or a tissue. Like BCH 711, it is intimately linked to BCH 709, Bioinformatics resources.

Contents

Proteome and proteomics, Protein 3D Structures, Protein Identification techniques (2-hybrid system, 2-D gel electrophoresis, Digestion analysis, Mass spectrometry/MALDI-TOF, MS/MS, Finger printing, Peptide and protein microarrays), Protein linkage mapping, Phosphorylome analysis, Mass spectrometry-based proteomics, 2-D gels and analysis, protein-protein interactions networks; Databases of information for protein sequences, structures, functions and interactions; Mining of protein databases, applications to human disease and agricultural studies.