Faculty of Science

Objectives

To present a detailed study of the structure and function of nucleic acids and mechanisms of gene expression as well as the concepts of recombinant DNA technology.

Contents

Gene expression and regulation; Replication, transcription and translation (protein synthesis); The operon and the regulation of gene expression in bacteria; Recombinant DNA Techniques: Cloning vectors, restriction enzymes and sites, transformation & transfection; Identification of recombinants; DNA sequencing methods, Polymerase chain reactions; Elements of Bioethics.