Faculty of Science

Objectives

To cover Functional Genomics and present it as the systematic analysis of gene activity or function in the context of normal or abnormal cellular or developmental processes through the development and application of genome-wide, high-throughput experimental methodologies.

Contents

The New Biology, Functional vs Comparative Genomics, Genome Mapping and organisation, Genome Sequencing projects, Assembling genome sequences, Sequence polymorphisms in genomes and SNPs, Differential Display, Gene Discovery – Expressed Sequencing Tags (ESTs); Serial Analysis of Gene Expression (SAGE) , Transcriptome analysis: cDNA microarrays, oligonucleotide microarrays, Regulation of Gene Expression, Metabolomics, Forward and Reverse Genetics, RNAi-mediated Gene Silencing, Gene Knockouts, Micro-RNAs and Small RNAs, Site-directed mutagenesis, Genomics as a systems biology, two-hybrid systems; Applications of genomics to medicine, agriculture, forensics and population studies.