Faculty of Science

Objectives

To provide hands-on experience and a critical appreciation of commonly employed analytical techniques in biochemistry and immunology

Contents

Quantitative determination of proteins, nucleic acids, sugars and lipids; Colorimetry, spectrophotometry, nephelometry, fluorimetry, chemilunescence and their applications in biochemical analyses; Quantitative gene amplification methods; Immunochemical Methods: Quantitative determination of humoral immune responses (antibodies): gel diffusion techniques, Enzyme immunoassays (ELISA), radio immuno-assay; Quantitative determination of cell-mediated immune responses: skin tests, cellular proliferation assays, cytokine responses; Radio-Isotopic Methods: Theory and practice of measuring beta and gamma radiation; Applications of radioisotopes in biology: labelling of cells and biomolecules; Spectroscopy: Principles and practice: UV, CD, ORD, NMR.

Emerging analytical methods: high throughput technologies.