Faculty of Science

Objectives

To provide a practical training that demonstrates many of the techniques described in Biochemical Methods I and elsewhere; Also to give students hands-on research and thesis writing experiences.

Contents

Experiments lasting up to a week or more will be carried out by students under supervision. Such experiments will involve a variety of separation and analytical methods, including ion-exchange and gel filtration chromatography, electrophoresis, SDS-PAGE, Western blotting, etc. Typical experiments include: Enzyme/protein purification, Km, Vmax and size determinations. Sub-cloning and expression of a recombinant clone, introducing DNA/RNA extraction/purification, PCR, hybridisation. Students will be required to report their results using a journal article format with citations.